Repair of the (6–4) Photoproduct by DNA Photolyase Requires Two Photons1. Issue 29 (12th June 2013)

• Record Type: Journal Article
• Authors: Yamamoto, Junpei; Martin, Ryan; Iwai, Shigenori; Plaza, Pascal; Brettel, Klaus
• Is Part Of: Angewandte Chemie international edition. Volume 52:Issue 29(2013)
• Page Start: 7432
• Page End: 7436
• Publication Date: 2013-06-12
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201301567
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>It takes two (photons) to tango</bold>: Single‐turnover flash experiments showed that the flavoenzyme (6–4) photolyase uses a successive two‐photon mechanism to repair the UV‐induced T(6–4)T lesion in DNA (see picture). The intermediate (X) formed by the first photoreaction is likely to be the oxetane‐bridged dimer T(ox)T. The enzyme could stabilize the normally short‐lived T(ox)T, allowing repair to be completed by the second photoreaction.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg270vv1c6" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>