The Structure of FemXWv in Complex with a Peptidyl‐RNA Conjugate: Mechanism of Aminoacyl Transfer from Ala‐tRNAAla to Peptidoglycan Precursors1. Issue 28 (6th June 2013)

• Record Type: Journal Article
• Authors: Fonvielle, Matthieu; Li de La Sierra‐Gallay, Inés; El‐Sagheer, Afaf H.; Lecerf, Maxime; Patin, Delphine; Mellal, Dénia; Mayer, Claudine; Blanot, Didier; Gale, Nittaya; Brown, Tom; van Tilbeurgh, Herman; Ethève‐Quelquejeu, Mélanie; Arthur, Michel
• Is Part Of: Angewandte Chemie international edition. Volume 52:Issue 28(2013)
• Page Start: 7278
• Page End: 7281
• Publication Date: 2013-06-06
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201301411
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>To gain insight</bold> into the catalytic mechanism of non‐ribosomal amino acid transferases, peptidyl‐RNA conjugates were synthesized for co‐crystallization with FemX_Wv of <italic>Weissella viridescens</italic>, which transfers <sc>L</sc>‐Ala from Ala‐tRNA^Ala to the peptidoglycan precursor UDP‐MurNAc‐pentapeptide. The structure of the resulting complex and mutational studies revealed the mechanism by which FemX_Wv binds its substrates for substrate‐assisted catalysis and stabilization of the tetrahedral intermediate.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg26mfvgb2" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>