Cover Picture: Expression of Fluorescent Cyclotides using Protein Trans‐Splicing for Easy Monitoring of Cyclotide–Protein Interactions (Angew. Chem. Int. Ed. 11/2013). Issue 11 (21st February 2013)

• Record Type: Journal Article
• Authors: Jagadish, Krishnappa; Borra, Radhika; Lacey, Vanessa; Majumder, Subhabrata; Shekhtman, Alexander; Wang, Lei; Camarero, Julio A.
• Is Part Of: Angewandte Chemie international edition. Volume 52:Issue 11(2013)
• Page Start: 3037
• Page End: 3037
• Publication Date: 2013-02-21
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201301068
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <italic> <bold>Cyclotides are natural microproteins</bold> </italic> found in plants with a head‐to‐tail circular Cys‐knotted topology. In their Communication on <ext-link ext-link-type="doi" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink">page 3126 ff., </ext-link> J. A. Camarero et al. show the recombinant production of a fluorescently labeled cyclotide inside live bacterial cells. Grown in the presence of <italic>p</italic>‐azidophenylalanine and a dibenzocyclooctyne derivative of the fluorescent dye aminomethylcoumarin acetate (AMCA) the peptide is labeled in vivo. Cover art: Isaac Mora.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg1v8wsv1c" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>