A Tryptophanyl‐tRNA Synthetase/tRNA Pair for Unnatural Amino Acid Mutagenesis in E. coli1. Issue 19 (2nd April 2013)

• Record Type: Journal Article
• Authors: Chatterjee, Abhishek; Xiao, Han; Yang, Peng‐Yu; Soundararajan, Gautam; Schultz, Peter G.
• Is Part Of: Angewandte Chemie international edition. Volume 52:Issue 19(2013)
• Page Start: 5106
• Page End: 5109
• Publication Date: 2013-04-02
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201301094
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>Selected</bold>: A tryptophanyl‐tRNA synthetase(TrpRS)/tRNA pair was evolved to genetically encode tryptophan analogues and other unnatural amino acids with large side chains in <italic>E. coli.</italic> A selection scheme was employed to identify TrpRS variants able to selectively charge tRNA_CUA^Trp with Trp analogues (see picture). Substitution of Trp66 in enhanced cyan fluorescent protein (ECFP) with these Trp analogues afforded ECFP variants with novel spectral properties.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg1zsxjnst" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>