Design and Synthesis of Minimalist Terminal Alkyne‐Containing Diazirine Photo‐Crosslinkers and Their Incorporation into Kinase Inhibitors for Cell‐ and Tissue‐Based Proteome Profiling1. Issue 33 (10th June 2013)

• Record Type: Journal Article
• Authors: Li, Zhengqiu; Hao, Piliang; Li, Lin; Tan, Chelsea Y. J.; Cheng, Xiamin; Chen, Grace Y. J.; Sze, Siu Kwan; Shen, Han‐Ming; Yao, Shao Q.
• Is Part Of: Angewandte Chemie international edition. Volume 52:Issue 33(2013)
• Page Start: 8551
• Page End: 8556
• Publication Date: 2013-06-10
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201300683
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>Less is more</bold>: A minimalist "clickable" photo‐crosslinker (see scheme) was incorporated with numerous small‐molecule kinase inhibitors. The resulting probes were used for both in vitro (cell lysates) and in situ (live cells) proteome profiling, for large‐scale identification of their potential cellular kinase targets and shows improved outcomes over previous probes.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg36jx3s9b" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>