A Genetically Encoded 19F NMR Probe for Tyrosine Phosphorylation1. Issue 14 (28th February 2013)

• Record Type: Journal Article
• Authors: Li, Fahui; Shi, Pan; Li, Jiasong; Yang, Fan; Wang, Tianyuan; Zhang, Wei; Gao, Feng; Ding, Wei; Li, Dong; Li, Juan; Xiong, Ying; Sun, Jinpeng; Gong, Weimin; Tian, Changlin; Wang, Jiangyun
• Is Part Of: Angewandte Chemie international edition. Volume 52:Issue 14(2013)
• Page Start: 3958
• Page End: 3962
• Publication Date: 2013-02-28
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201300463
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>Simple and selective</bold>: Tyrosine phosphorylation is a pivotal post‐translational modification which regulates the enzymatic activity, protein conformation, and protein–protein interactions. The highly efficient genetic incorporation of 3, 5‐difluorotyrosine (F2Y) in <italic>E. coli</italic> and the use of F2Y as a ¹⁹F NMR probe for the tyrosine phosphorylation are reported (see picture).<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg1x47k7kk" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>