Two‐Color Glycan Labeling of Live Cells by a Combination of Diels–Alder and Click Chemistry1. Issue 15 (6th March 2013)

• Record Type: Journal Article
• Authors: Niederwieser, Andrea; Späte, Anne‐Katrin; Nguyen, Long Duc; Jüngst, Christian; Reutter, Werner; Wittmann, Valentin
• Is Part Of: Angewandte Chemie international edition. Volume 52:Issue 15(2013)
• Page Start: 4265
• Page End: 4268
• Publication Date: 2013-03-06
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201208991
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>One is not enough</bold>: Terminal alkenes are used as chemical reporters and ligation partners for 1, 2, 4, 5‐tetrazines in a Diels–Alder reaction with inverse electron demand (DARinv). Combination with strain‐promoted azide–alkyne cycloaddition (SPAAC) allows the visualization of two different glycan structures in one experiment.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg1x47fbpv" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>