Expression of Fluorescent Cyclotides using Protein Trans‐Splicing for Easy Monitoring of Cyclotide–Protein Interactions1. Issue 11 (15th January 2013)

• Record Type: Journal Article
• Authors: Jagadish, Krishnappa; Borra, Radhika; Lacey, Vanessa; Majumder, Subhabrata; Shekhtman, Alexander; Wang, Lei; Camarero, Julio A.
• Is Part Of: Angewandte Chemie international edition. Volume 52:Issue 11(2013)
• Page Start: 3126
• Page End: 3131
• Publication Date: 2013-01-15
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201209219
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>Going in circles</bold>: Expression of cyclotides containing non‐natural amino acids (see scheme; gray sphere) inside live bacterial cells was accomplished using a highly efficient split intein in combination with nonsense codon suppressor tRNA technology. Cyclotides containing <italic>p</italic>‐azidophenylalanine can be expressed in live bacterial cells and easily labeled using copper‐free click chemistry to monitor the cyclotide–protein interactions.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg1v8wsvh3" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>