Liquid AP‐UV‐MALDI Enables Stable Ion Yields of Multiply Charged Peptide and Protein Ions for Sensitive Analysis by Mass Spectrometry1. Issue 8 (22nd January 2013)

• Record Type: Journal Article
• Authors: Cramer, Rainer; Pirkl, Alexander; Hillenkamp, Franz; Dreisewerd, Klaus
• Is Part Of: Angewandte Chemie international edition. Volume 52:Issue 8(2013)
• Page Start: 2364
• Page End: 2367
• Publication Date: 2013-01-22
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201208628
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>All charged up</bold>: High and prolonged yields of multiply charged peptide and protein ions can be formed in MALDI mass spectrometry using liquid UV‐MALDI matrices and a heated ion‐transfer tube. The key features are low laser energies of 1–10 μJ, resulting in fluences of less than 200 to 2000 J m⁻² and low sample ablation, high sensitivity, and continuous ion generation over tens of thousands of laser shots.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg1xm2h0gw" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>