Combinatorial Nucleoside‐Deletion‐Scanning Mutagenesis of Functional DNA1. Issue 10 (1st February 2013)

• Record Type: Journal Article
• Authors: Samanta, Biswajit; Höbartner, Claudia
• Is Part Of: Angewandte Chemie international edition. Volume 52:Issue 10(2013)
• Page Start: 2995
• Page End: 2999
• Publication Date: 2013-02-01
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201208103
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>Compact and informative</bold>: Individual nucleosides are statistically mutated by replacement with a non‐nucleosidic spacer unit Δ that encodes the "deletion" in a single DNA library. This efficient mutagenesis approach enables minimization of functional DNA, identifying essential nucleotides, as demonstrated for two nucleic‐acid ligating deoxyribozymes.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg1ts9s4x2" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>