Rewiring Translation for Elongation Factor Tu‐Dependent Selenocysteine Incorporation1. Issue 5 (27th November 2012) - Aldag, Caroline
Abstract Enjoying UTu in concert: A synthetic tRNA (tRNAUTu) was used as a substrate for three E. coli proteins: seryl‐tRNA synthetase (SerRS) forming Ser‐tRNAUTu, selenocysteine (Sec) synthase (SelA) generating Sec‐tRNAUTu, and EF‐Tu for Sec‐tRNAUTu transport to the ribosome (see scheme). tRNAUTu can be used by the ribosome, thus allowing site‐specific Sec insertion into proteins, including formate dehydrogenase H, selenoglutaredoxin, and glutathione peroxidase.