A Gel‐Encapsulated Bioreactor System for NMR Studies of Protein–Protein Interactions in Living Mammalian Cells1. Issue 4 (29th November 2012)

• Record Type: Journal Article
• Authors: Kubo, Satoshi; Nishida, Noritaka; Udagawa, Yuko; Takarada, Osamu; Ogino, Shinji; Shimada, Ichio
• Is Part Of: Angewandte Chemie international edition. Volume 52:Issue 4(2013)
• Page Start: 1208
• Page End: 1211
• Publication Date: 2012-11-29
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201207243
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>Staying alive</bold>: The major limitation of in‐cell NMR spectroscopy methods is the occurrence of cell death during the NMR measurement. To overcome this problem, a bioreactor was utilized that can perfuse the cells in the NMR tube, thereby maintaining the conditions for more than 5 h. By using the bioreactor, the binding site of an externally introduced protein for an endogenous molecule in HeLa S3 cells was identified.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg1pjpmxsv" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>