Β2‐Adrenergic Receptor Solutions for Structural Biology Analyzed with Microscale NMR Diffusion Measurements1. Issue 1 (24th October 2012)

• Record Type: Journal Article
• Authors: Horst, Reto; Stanczak, Pawel; Stevens, Raymond C.; Wüthrich, Kurt
• Is Part Of: Angewandte Chemie international edition. Volume 52:Issue 1(2013)
• Page Start: 331
• Page End: 335
• Publication Date: 2012-10-24
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201205474
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>Microcoil NMR measurements</bold> were performed to determine the final composition of solutions of the β₂‐adrenergic receptor (β₂AR) reconstituted with a detergent and to study the hydrodynamic properties of the detergent micelles containing β₂AR (see picture). Standards are established for the reproducible preparation of G‐protein‐coupled receptor solutions for crystallization trials and solution NMR studies.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg1pjrq2g9" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>