A Switchable Two‐Photon Membrane Tracer Capable of Imaging Membrane‐Associated Protein Tyrosine Phosphatase Activities1. Issue 1 (4th October 2012)

• Record Type: Journal Article
• Authors: Li, Lin; Shen, Xiaoqin; Xu, Qing‐Hua; Yao, Shao Q.
• Is Part Of: Angewandte Chemie international edition. Volume 52:Issue 1(2013)
• Page Start: 424
• Page End: 428
• Publication Date: 2012-10-04
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201205940
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>When I look into your cells</bold>: A two‐photon dye (<bold>Flu7</bold>) was developed (see scheme), which strongly fluoresces only upon selective binding to the plasma membrane of mammalian cells. Upon addition of a UV‐ and phosphatase‐controlled quencher (<bold>Q12</bold>), the system exhibits ON/OFF/ON fluorescence switching and can be used to image membrane‐associated receptor‐like protein tyrosine phosphatase (RPTP) activity.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg1pjrq1nj" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>