Novel approach for the development of axenic microalgal cultures from environmental samples. Issue 4 (9th July 2013)

• Record Type: Journal Article
• Authors: Cho, Dae‐Hyun; Ramanan, Rishiram; Kim, Byung‐Hyuk; Lee, Jimin; Kim, Sora; Yoo, Chan; Choi, Gang‐Guk; Oh, Hee‐Mock; Kim, Hee‐Sik; Lindell, D.
• Is Part Of: Journal of phycology. Volume 49:Issue 4(2013:Aug.)
• Page Start: 802
• Page End: 810
• Publication Date: 2013-07-09
• Subjects: Algae -- Periodicals; 579.8
• DOI: 10.1111/jpy.12091
• Languages: English
• ISSNs: 0022-3646

Abstract

<abstract abstract-type="main" id="jpy12091-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p>We demonstrated a comprehensive approach for development of axenic cultures of microalgae from environmental samples. A combination of ultrasonication, fluorescence‐activated cell sorting (FACS), and micropicking was used to isolate axenic cultures of <italic>Chlorella vulgaris </italic>Beyerinck (Beijerinck) and <italic>Chlorella sorokiniana</italic> Shihira &amp; R.W. Krauss from swine wastewater, and <italic>Scenedesmus</italic> sp. YC001 from an open pond. Ultrasonication dispersed microorganisms attached to microalgae and reduced the bacterial population by 70%, and when followed by cell sorting yielded 99.5% pure microalgal strains. The strains were rendered axenic by the novel method of micropicking and were tested for purity in both solid and liquid media under different trophic states. Denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene confirmed the absence of unculturable bacteria, whereas fluorescence microscopy and scanning electron microscopy (SEM) further confirmed the axenicity. This is the most comprehensive approach developed to date for obtaining axenic microalgal strains without the use of antibiotics and repetitive subculturing.</p> </abstract>