Activation of GABAA receptors suppresses ethanol‐induced upregulation of type 1 IP3 receptors. Issue 1 (16th October 2012)

• Record Type: Journal Article
• Authors: Mizuno, Koji; Kurokawa, Kazuhiro; Ohkuma, Seitaro
• Is Part Of: Synapse. Volume 67:Issue 1(2013:Jan.)
• Page Start: 51
• Page End: 55
• Publication Date: 2012-10-16
• Subjects: Synapses -- Periodicals; 612
• DOI: 10.1002/syn.21610
• Languages: English
• ISSNs: 0887-4476

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Although Type 1 inositol 1, 4, 5‐trisphosphate receptors (IP₃Rs‐1) are one of the major calcium channels to regulate intracellular Ca²⁺ concentration, there have been few available data how their expression is modified by long‐term exposure to ethanol. The present study attempted to clarify mechanisms of modification of IP₃R‐1 expression during long‐term ethanol exposure by γ‐aminobutyric acid (GABA)A receptors using mouse cerebral cortical neurons. Long‐term exposure to ethanol induced IP₃R‐1 protein upregulation following increased expression of its mRNA. Pretreatment with muscimol, a selective GABA_A receptor agonist, significantly suppressed the ethanol‐induced upregulation of IP₃R‐1 protein and its mRNA, which was significantly abolished by bicuculline, a selective GABA_A receptor antagonist. These results indicate that GABA_A receptors negatively regulate the ethanol‐induced upregulation of IP₃R‐1 protein expression via the suppression of gene transcription. Synapse, 2013. © 2012 Wiley Periodicals, Inc.</p> </abstract>