A Molecular Beacon‐Based Signal‐Off Surface‐Enhanced Raman Scattering Strategy for Highly Sensitive, Reproducible, and Multiplexed DNA Detection. Issue 15 (29th January 2013)

• Record Type: Journal Article
• Authors: Wei, Xinpan; Su, Shao; Guo, Yuanyuan; Jiang, Xiangxu; Zhong, Yiling; Su, Yuanyuan; Fan, Chunhai; Lee, Shuit‐Tong; He, Yao
• Is Part Of: Small. Volume 9:Issue 15(2013:Aug.)
• Page Start: 2493
• Page End: 2499
• Publication Date: 2013-01-29
• Subjects: Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.201202914
• Languages: English
• ISSNs: 1613-6810

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>Stem‐loop oligonucleotides tagged with organic dyes</bold> are employed as capture and reporter probes in a molecular beacon‐based signal‐off surface‐ enhanced Raman scattering (SERS) strategy. DNA strands are immobilized on a gold nanoparticle‐decorated silicon nanowire array (AuNPs@SiNWAr). DNA with a concentration down to ≈10 f<sc>M</sc> is detected under irradiation from a low‐power laser, which is better than or comparable to that detected by signal‐on SERS methods. The signal‐off strategy allows identification of single‐base mismatches and detection of multiplexed DNA. <boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg37ntgvkc" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>