Nanoparticle Transport in Epithelial Cells: Pathway Switching Through Bioconjugation. Issue 19 (2nd May 2013)

• Record Type: Journal Article
• Authors: Fowler, Robyn; Vllasaliu, Driton; Trillo, Francisco Fernández; Garnett, Martin; Alexander, Cameron; Horsley, Helen; Smith, Bryan; Whitcombe, Ian; Eaton, Mike; Stolnik, Snow
• Is Part Of: Small. Volume 9:Issue 19(2013:Oct.)
• Page Start: 3282
• Page End: 3294
• Publication Date: 2013-05-02
• Subjects: Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.201202623
• Languages: English
• ISSNs: 1613-6810

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>The understanding and control of nanoparticle transport into and through cellular compartments is central to biomedical applications of nanotechnology. Here, it is shown that the transport pathway of 50 nm polystyrene nanoparticles decorated with vitamin B₁₂ in epithelial cells is different compared to both soluble B₁₂ ligand and unmodified nanoparticles, and this is not attributable to B₁₂ recognition alone. Importantly, the study indicates that vitamin B₁₂‐conjugated nanoparticles circumnavigate the lysosomal compartment, the destination of soluble vitamin B₁₂ ligand. Whereas cellular trafficking of soluble B₁₂ is confirmed to occur via the clathrin‐mediated pathway, transport of B₁₂‐conjugated nanoparticles appears to predominantly take place by a route that is perturbed by caveolae‐specific inhibitors. This data suggests that, following its conjugation to nanoparticles, in addition to dramatically increasing the cellular uptake of nanoparticles, the normal cell trafficking of B₁₂ is switched to an alternative pathway, omitting the lysosomal stage: a result with important implications for oral delivery of nanoparticulate diagnostics and therapeutics.</p> </abstract>