Short‐term effect of cryotherapy on human scleral tissue by atomic force microscopy. Issue 5 (5th December 2012)

• Record Type: Journal Article
• Authors: Lee, Seung Jun; Choi, Samjin; Kim, Moo Sang; Cheong, Youjin; Kwak, Hyung‐Woo; Park, Hun‐Kuk; Jin, Kyung‐Hyun
• Is Part Of: Scanning. Volume 35:Issue 5(2013:Sep./Oct.)
• Page Start: 302
• Page End: 307
• Publication Date: 2012-12-05
• Subjects: Scanning electron microscopy -- Periodicals; 502.825
• DOI: 10.1002/sca.21068
• Languages: English
• ISSNs: 0161-0457

Abstract

<abstract abstract-type="main"> <title>Summary</title> <p>This study investigated the inflammatory effect of cryotherapy application on collagen matrix network in human infant sclera. Donor scleral tissues taken from three infant patients divided into five groups: control group, sham‐treated group, and three cryotreated groups. In the cryotherapy groups, the sclera was treated for 5 s, 10 s, and 20 s with −80°C freezing by a cryosurgical system. The cryotreated reactions were examined using double histological analysis with hematoxylin‐eosin and Masson's trichrome, and atomic force microscopy analysis to quantify the diameter and D‐banding of collagen fibrils. The infant scleral tissues treated with cryotherapy showed a significantly increased collagen density associated with inflammatory response (<italic>p</italic> &lt; 0.05), increased fibril diameter (<italic>p</italic> &lt; 0.005) compared to the scleral tissues in the control group. The results directly suggest that the cryotherapy affects the morphology of scleral collagen. SCANNING 35:302‐307, 2013. © 2012 Wiley Periodicals, Inc.</p> </abstract>