Metabolic responses of Escherichia coli upon glucose pulses captured by a capacitive field‐effect sensor. Issue 5 (2nd May 2013)

• Record Type: Journal Article
• Authors: Huck, Christina; Schiffels, Johannes; Herrera, Cony N.; Schelden, Maximilian; Selmer, Thorsten; Poghossian, Arshak; Baumann, Marcus E. M.; Wagner, Patrick; Schöning, Michael J.
• Is Part Of: Physica status solidi. Volume 210:Issue 5(2013:May)
• Page Start: 926
• Page End: 931
• Publication Date: 2013-05-02
• Subjects: Solid state physics -- Periodicals; Solids -- Industrial applications -- Periodicals; 530.41
• DOI: 10.1002/pssa.201200900
• Languages: English
• ISSNs: 1862-6300

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="pssa201200900-sec-0001" sec-type="section"> <p>Living cells are complex biological systems transforming metabolites taken up from the surrounding medium. Monitoring the responses of such cells to certain substrate concentrations is a challenging task and offers possibilities to gain insight into the vitality of a community influenced by the growth environment. Cell‐based sensors represent a promising platform for monitoring the metabolic activity and thus, the "welfare" of relevant organisms. In the present study, metabolic responses of the model bacterium <italic>Escherichia coli</italic> in suspension, layered onto a capacitive field‐effect structure, were examined to pulses of glucose in the concentration range between 0.05 and 2 mM. It was found that acidification of the surrounding medium takes place immediately after glucose addition and follows Michaelis–Menten kinetic behavior as a function of the glucose concentration. In future, the presented setup can, therefore, be used to study substrate specificities on the enzymatic level and may as well be used to perform investigations of more complex metabolic responses. Conclusions and perspectives highlighting this system are discussed.</p> </sec> </abstract>