Enrichment strategies in glycomics‐based lung cancer biomarker development. Issue 9 (6th August 2013)

• Record Type: Journal Article
• Authors: Ruhaak, L. Renee; Nguyen, Uyen Thao; Stroble, Carol; Taylor, Sandra L.; Taguchi, Ayumu; Hanash, Samir M.; Lebrilla, Carlito B.; Kim, Kyoungmi; Miyamoto, Suzanne; Pierce, Michael; Taniguchi, Naoyuki
• Is Part Of: Proteomics. Volume 7:Issue 9/10(2013)
• Page Start: 664
• Page End: 676
• Publication Date: 2013-08-06
• Subjects: Proteomics -- Periodicals; 572.605
• DOI: 10.1002/prca.201200131
• Languages: English
• ISSNs: 1862-8346

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="prca1472-sec-0010" sec-type="section"> <title>Purpose</title> <p>There is a need to identify better glycan biomarkers for diagnosis, early detection, and treatment monitoring in lung cancer using biofluids such as blood. Biofluids are complex mixtures of proteins dominated by a few high abundance proteins that may not have specificity for lung cancer. Therefore, two methods for protein enrichment were evaluated; affinity capturing of IgG and enrichment of medium abundance proteins, thus allowing us to determine which method yields the best candidate glycan biomarkers for lung cancer.</p> </sec> <sec id="prca1472-sec-0020" sec-type="section"> <title>Experimental design</title> <p> <italic>N</italic>‐glycans isolated from plasma samples from 20 cases of lung adenocarcinoma and 20 matched controls were analyzed using nLC‐PGC‐chip‐TOF‐MS (where PGC is porous‐graphitized carbon). <italic>N</italic>‐glycan profiles were obtained for five different fractions: total plasma, isolated IgG, IgG‐depleted plasma, and the bound and flow‐through fractions of protein enrichment.</p> </sec> <sec id="prca1472-sec-0030" sec-type="section"> <title>Results</title> <p>Four glycans differed significantly (false discovery rate, FDR < 0.05) between cases and controls in whole unfractionated plasma, while four other glycans differed significantly by cancer status in the IgG fraction. No significant glycan differences were observed in the other fractions.</p> </sec> <sec id="prca1472-sec-0040" sec-type="section"> <title>Conclusions and clinical relevance</title> <p>These results confirm that the <italic>N</italic>‐glycan profile in plasma of lung cancer patients is different from healthy controls and appears to be dominated by alterations in relatively abundant proteins.</p> </sec> </abstract>