ITRAQ‐based quantitative proteomic analysis reveals new metabolic pathways of wheat seedling growth under hydrogen peroxide stress. Issue 20 (13th September 2013)

• Record Type: Journal Article
• Authors: Ge, Pei; Hao, Pengchao; Cao, Min; Guo, Guangfang; Lv, Dongwen; Subburaj, Saminathan; Li, Xiaohui; Yan, Xing; Xiao, Jitian; Ma, Wujun; Yan, Yueming
• Is Part Of: Proteomics. Volume 13:Issue 20(2013:Oct.)
• Page Start: 3046
• Page End: 3058
• Publication Date: 2013-09-13
• Subjects: Proteins -- Separation -- Periodicals; Bioinformatics -- Periodicals; Proteomics -- Periodicals; Genomes -- Periodicals; Molecular genetics -- Periodicals; 572.605
• DOI: 10.1002/pmic.201300042
• Languages: English
• ISSNs: 1615-9853

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>As an abundant ROS, hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) plays pivotal roles in plant growth and development. In this work, we conducted for the first time an iTRAQ‐based quantitative proteomic analysis of wheat seedling growth under different exogenous H<sub>2</sub>O<sub>2</sub> treatments. The growth of seedlings and roots was significantly restrained by increased H<sub>2</sub>O<sub>2</sub> concentration stress. Malondialdehyde, soluble sugar, and proline contents as well as peroxidase activity increased with increasing H<sub>2</sub>O<sub>2</sub> levels. A total of 3 425 proteins were identified by iTRAQ, of which 157 showed differential expression and 44 were newly identified H<sub>2</sub>O<sub>2</sub>‐responsive proteins. H<sub>2</sub>O<sub>2</sub>‐responsive proteins were mainly involved in stress/defense/detoxification, signal transduction, and carbohydrate metabolism. It is clear that up‐regulated expression of signal transduction and stress/defence/detoxification‐related proteins under H<sub>2</sub>O<sub>2</sub> stress, such as plasma membrane intrinsic protein 1, fasciclin‐like arabinogalactan protein, and superoxide dismutase, could contribute to H<sub>2</sub>O<sub>2</sub> tolerance of wheat seedlings. Increased gluconeogenesis (phosphoenol‐pyruvate carboxykinase) and decreased pyruvate kinase proteins are potentially related to the higher H<sub>2</sub>O<sub>2</sub> tolerance of wheat seedlings. A metabolic pathway of wheat seedling growth under H<sub>2</sub>O<sub>2</sub> stress is presented.</p> </abstract>