Adaptation of Phaeobacter inhibens DSM 17395 to growth with complex nutrients. Issue 18 (5th July 2013)

• Record Type: Journal Article
• Authors: Zech, Hajo; Hensler, Michael; Koßmehl, Sebastian; Drüppel, Katharina; Wöhlbrand, Lars; Trautwein, Kathleen; Hulsch, Reiner; Maschmann, Uwe; Colby, Thomas; Schmidt, Jürgen; Reinhardt, Richard; Schmidt‐Hohagen, Kerstin; Schomburg, Dietmar; Rabus, Ralf; Rabus, Ralf
• Is Part Of: Proteomics. Volume 13:Issue 18/19(2013:Oct.)
• Page Start: 2851
• Page End: 2868
• Publication Date: 2013-07-05
• Subjects: Proteins -- Separation -- Periodicals; Bioinformatics -- Periodicals; Proteomics -- Periodicals; Genomes -- Periodicals; Molecular genetics -- Periodicals; 572.605
• DOI: 10.1002/pmic.201200513
• Languages: English
• ISSNs: 1615-9853

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <italic>Phaeobacter inhibens</italic> DSM 17395, a member of the <italic>Roseobacter</italic> clade, was studied for its adaptive strategies to complex and excess nutrient supply, here mimicked by cultivation with Marine Broth (MB). During growth in process‐controlled fermenters, <italic>P</italic>. <italic>inhibens</italic> DSM 17395 grew faster (3.6‐fold higher μ<sub>max</sub>) and reached higher optical densities (2.2‐fold) with MB medium, as compared to the reference condition of glucose‐containing mineral medium. Apparently, in the presence of MB medium, metabolism was tuned to maximize growth rate at the expense of efficiency. Comprehensive proteomic analysis of cells harvested at ½ OD<sub>max</sub> identified 1783 (2D DIGE, membrane and extracellular protein‐enriched fractions, shotgun) different proteins (50.5% coverage), 315 (based on 2D DIGE) of which displayed differential abundance profiles. Moreover, 145 different metabolites (intra‐ and extracellular combined) were identified, almost all of which (140) showed abundance changes. During growth with MB medium, <italic>P</italic>. <italic>inhibens</italic> DSM 17395 specifically formed the various proteins required for utilization of phospholipids and several amino acids, as well as for gluconeogenesis. Metabolic tuning on amino acid utilization is also reflected by massive discharge of urea to dispose the cell of excess ammonia. Apparently, <italic>P</italic>. <italic>inhibens</italic> DSM 17395 modulated its metabolism to simultaneously utilize diverse substrates from the complex nutrient supply.</p> </abstract>