From spots to beads—PTM‐peptide bead arrays for the characterization of anti‐histone antibodies. Issue 6 (11th March 2013)

• Record Type: Journal Article
• Authors: Heubach, Yvonne; Planatscher, Hannes; Sommersdorf, Cornelia; Maisch, Daniel; Maier, Julia; Joos, Thomas O.; Templin, Markus F.; Poetz, Oliver; Zahedi, René; Sickmann, Albert
• Is Part Of: Proteomics. Volume 13:Issue 6(2013:Mar.)
• Page Start: 1010
• Page End: 1015
• Publication Date: 2013-03-11
• Subjects: Proteins -- Separation -- Periodicals; Bioinformatics -- Periodicals; Proteomics -- Periodicals; Genomes -- Periodicals; Molecular genetics -- Periodicals; 572.605
• DOI: 10.1002/pmic.201200383
• Languages: English
• ISSNs: 1615-9853

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Antibodies that recognize PTMs of histones play a central role in epigenetic proteomic research. Modification‐specific antibodies are employed in chromatin immunoprecipitation, for Western blotting and during the immunoprecipitation steps for MS‐based global proteomic analyses. Knowledge about the antibodies' off‐target binding is essential for the interpretation of experimental data. To address this challenge we developed a fast and cost efficient system for generating peptide bead arrays. We employed this method to establish a bead‐based peptide array containing 384 peptides displaying phosphorylated, acetylated, methylated, and citrullinated N‐terminal regions of histones H2A, H2B, H3 and H4 and controls. We profiled the binding of 40 PTM‐specific antibodies important for epigenetic proteomic research.</p> </abstract>