Proteomic identification of Listeria monocytogenes surface‐associated proteins. Issue 20 (8th October 2013)

• Record Type: Journal Article
• Authors: Zhang, Cathy X. Y.; Creskey, Marybeth C.; Cyr, Terry D.; Brooks, Brian; Huang, Hongsheng; Pagotto, Franco; Lin, Min
• Is Part Of: Proteomics. Volume 13:Issue 20(2013:Oct.)
• Page Start: 3040
• Page End: 3045
• Publication Date: 2013-10-08
• Subjects: Proteins -- Separation -- Periodicals; Bioinformatics -- Periodicals; Proteomics -- Periodicals; Genomes -- Periodicals; Molecular genetics -- Periodicals; 572.605
• DOI: 10.1002/pmic.201200449
• Languages: English
• ISSNs: 1615-9853

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>This study aimed to identify proteins exposed on the surface of <italic>Listeria monocytogenes</italic> cells for diagnostic reagent development. Brief trypsin treatment of <italic>L. monocytogenes</italic> cells followed by peptide separation and identification by nano‐LC and online‐MS/MS was performed. In parallel, as a negative control, proteins secreted into the digest buffer as well as proteins from cell lysis were identified. One hundred and seventy‐four proteins were identified in at least two of three trials in either the negative control or during cell digest. Nineteen surface, 21 extracellularly secreted, 132 cytoplasmic, and two phage proteins were identified. Immunofluorescence microscopy of <italic>L. monocytogenes</italic> cells revealed the surface localization of two potential candidates for <italic>L. monocytogenes</italic> isolation and detection: lipoprotein LMOf2365_0546 and PBPD1 (LMOf2365_2742). In this report, we present the first data set of surface‐exposed <italic>L. monocytogenes</italic> proteins currently available. The data have been deposited to the ProteomeXchange Consortium with identifier PXD000035.</p> </abstract>