Tissue proteomics of the low‐molecular weight proteome using an integrated cLC‐ESI‐QTOFMS approach. Issue 9 (5th April 2013)

• Record Type: Journal Article
• Authors: Alvarez, MeiHwa Tanielle Bench; Shah, Dipti Jigar; Thulin, Craig D.; Graves, Steven W.
• Is Part Of: Proteomics. Volume 13:Issue 9(2013:May)
• Page Start: 1400
• Page End: 1411
• Publication Date: 2013-04-05
• Subjects: Proteins -- Separation -- Periodicals; Bioinformatics -- Periodicals; Proteomics -- Periodicals; Genomes -- Periodicals; Molecular genetics -- Periodicals; 572.605
• DOI: 10.1002/pmic.201200291
• Languages: English
• ISSNs: 1615-9853

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Analysis of the protein/peptide composition of tissue has provided meaningful insights into tissue biology and even disease mechanisms. However, little has been published regarding top down methods to investigate lower molecular weight (MW) (500–5000 Da) species in tissue. Here, we evaluate a tissue proteomics approach involving tissue homogenization followed by depletion of large proteins and then cLC‐MS (where c stands for capillary) analysis to interrogate the low MW/low abundance tissue proteome. In the development of this method, sheep heart, lung, liver, kidney, and spleen were surveyed to test our ability to observe tissue differences. After categorical tissue differences were demonstrated, a detailed study of this method's reproducibility was undertaken to determine whether or not it is suitable for analyzing more subtle differences in the abundance of small proteins and peptides. Our results suggest that this method should be useful in exploring the low MW proteome of tissues.</p> </abstract>