Shotgun proteomic analysis of two Bartonella quintana strains. Issue 8 (15th March 2013)

• Record Type: Journal Article
• Authors: Fabietti, Alessandra; Gaspari, Marco; Krishnan, Shibu; Quirino, Angela; Liberto, Maria Carla; Cuda, Giovanni; Focà, Alfredo; Figeys, Daniel
• Is Part Of: Proteomics. Volume 13:Issue 8(2013:Apr.)
• Page Start: 1375
• Page End: 1378
• Publication Date: 2013-03-15
• Subjects: Proteins -- Separation -- Periodicals; Bioinformatics -- Periodicals; Proteomics -- Periodicals; Genomes -- Periodicals; Molecular genetics -- Periodicals; 572.605
• DOI: 10.1002/pmic.201200165
• Languages: English
• ISSNs: 1615-9853

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>This study reports the first extensive shotgun analysis of the <italic>Bartonella quintana</italic> proteome. Proteins extracted from two <italic>B. quintana</italic> strains, Oklahoma and JK31, were analyzed in triplicate analyses by a bottom‐up approach consisting of tryptic digestion in SDS‐containing buffer, strong cation‐exchange StageTip fractionation, and nano‐LC‐MS/MS analysis. By setting spectral false discovery rate below 0.5%, 548 unique proteins were identified overall, of which 409 protein identifications were shared between the two strains. The data set, which achieves the highest proteome coverage for <italic>B. quintana</italic> to date, could be exploited for the quantitative analysis of a selected subset of target proteins.</p> </abstract>