Protective effect of cyanidin‐3‐O‐β‐D‐glucopyranoside fraction from mulberry fruit pigment against oxidative damage in streptozotocin‐induced diabetic rat bladder12. Issue 5 (5th November 2012)

• Record Type: Journal Article
• Authors: Ha, U‐Syn; Bae, Woong‐Jin; Kim, Su‐Jin; Yoon, Byung‐Il; Jang, Hoon; Hong, Sung‐Hoo; Lee, Ji‐Yeoul; Hwang, Seung‐Yeon; Kim, Sae‐Woong
• Is Part Of: Neurourology and urodynamics. Volume 32:Issue 5(2013:Jun.)
• Page Start: 493
• Page End: 499
• Publication Date: 2012-11-05
• Subjects: Urinary organs -- Periodicals; Urodynamics -- Periodicals; Urology -- Periodicals; 616.6
• DOI: 10.1002/nau.22334
• Languages: English
• ISSNs: 0733-2467

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="abs1-1" sec-type="section"> <title>Aims</title> <p>To determine whether cyanidin‐3‐<italic>O</italic>‐β‐<sc>D</sc>‐glucopyranoside (C3G) fraction from mulberry fruit pigment has protective effects against bladder dysfunction on streptozotocin‐induced diabetic rats</p> </sec> <sec id="abs1-2" sec-type="section"> <title>Methods</title> <p>Sprague–Dawley rats were divided into three groups (n = 12 in each): normal, diabetes (DM), and DM treated with C3G fraction (DM + C3G). The DM and DM + C3G groups received a single injection of streptozotocin (50 mg/kg) intraperitoneally. Four weeks after the induction of diabetes, the DM + C3G group was treated with daily oral C3G (10 mg/kg) dissolved in water, for 8 weeks. After 12 weeks of streptozotocin injections, rats in each group underwent cystometrography and bladders were used for evaluation of apoptosis and oxidative stress.</p> </sec> <sec id="abs1-3" sec-type="section"> <title>Results</title> <p>The DM group showed a markedly lower maximal intravesical pressure than that observed in the control group, whereas rats in the DM + C3G group showed improved maximum intravesical pressure associated with minimization of apoptosis, and increased levels of Akt and Bad phosphorylation, implying inhibition of pro‐apoptotic stimuli. The level of 8‐hydroxy‐2‐deoxyguanosine, a marker of oxidative stress, was significantly greater in the DM group compared to the control group and it was significantly reduced in the C3G treated group. Immunoblotting revealed a significant decrease in the levels of the superoxide dismutase protein and nerve growth factor in the DM group compared with the control group; however, these proteins were upregulated in the DM + C3G group compared with the DM group.</p> </sec> <sec id="abs1-4" sec-type="section"> <title>Conclusions</title> <p>The study is the first to suggest that C3G fraction have a potency to protect the bladder under conditions of diabetes‐induced oxidative stress. Neurourol. Urodynam. 32: 493–499, 2013. © 2012 Wiley Periodicals, Inc.</p> </sec> </abstract>