Folic acid supplementation use and the MTHFR C677T polymorphism in orofacial clefts etiology: An individual participant data pooled‐analysis. Issue 8 (13th May 2013)

• Record Type: Journal Article
• Authors: Butali, Azeez; Little, Julian; Chevrier, Cécile; Cordier, Sylvian; Steegers‐Theunissen, Regine; Jugessur, Astanand; Oladugba, Bola; Mossey, Peter A.
• Is Part Of: Birth defects research. Volume 97:Issue 8(2013:Aug.)
• Page Start: 509
• Page End: 514
• Publication Date: 2013-05-13
• Subjects: Teratology -- Periodicals; Abnormalities, Human -- Research -- Periodicals; Abnormalities, Human -- Periodicals; 616.043
• DOI: 10.1002/bdra.23133
• Languages: English
• ISSNs: 1542-0752

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="bdra23133-sec-0001" sec-type="section"> <title>BACKGROUND</title> <p>This study examines gene–environment interaction between the <italic>MTHFR</italic> C667T polymorphism and folic acid in the etiology of orofacial clefts (OFC). We used a pooled‐analytical approach on four studies that used similar methods.</p> </sec> <sec id="bdra23133-sec-0002" sec-type="section"> <title>METHODS</title> <p>We used logistic regression to analyze the pooled sample of 1149 isolated cases and 1161 controls. Fetal and maternal <italic>MTHFR</italic> C677T genotypes, and maternal periconceptional exposure to smoking, alcohol, vitamin containing folic acid and folic acid supplements were contrasted between the cleft types [non‐syndromic clefts lip or without cleft palate (CL(P)) and non‐syndromic cleft palate (CP)] and control groups.</p> </sec> <sec id="bdra23133-sec-0003" sec-type="section"> <title>RESULTS</title> <p>There was a reduced risk of CL(P) with maternal folic acid use (<italic>p</italic> = 0.008; OR = 0.70, 95% CI: 0.65–0.94) and with supplements containing folic acid (<italic>p</italic> = 0.028, OR = 0.80, 95% CI: 0.65–0.94). Maternal smoking increased the risk of both CL(P) (<italic>p</italic> < 10 <italic>e−</italic>3; OR = 1.62, 95% CI: 1.35–1.95) and CP (<italic>p</italic> = 0.028; OR = 1.38, 95% CI: 1.04–1.83). No significant risk was observed with either maternal or fetal <italic>MTHFR</italic> C677T genotypes.</p> </sec> <sec id="bdra23133-sec-0004" sec-type="section"> <title>CONCLUSION</title> <p>This individual participant data (IPD) meta‐analysis affords greater statistical power and can help alleviate the problems associated with aggregate‐level data‐sharing. The result of this IPD meta‐analysis is consistent with previous reports suggesting that folic acid and smoking influence OFC outcomes. <italic>Birth Defects Research (Part A) 97:509‐514, 2013</italic>. © 2013 Wiley Periodicals, Inc.</p> </sec> </abstract>