Labeling of the pathogenic bacterium Staphylococcus aureus with gold or ferric oxide‐core nanoparticles highlights new capabilities for investigation of host–pathogen interactions. Issue 2 (17th December 2013)

• Record Type: Journal Article
• Authors: Depke, Maren; Surmann, Kristin; Hildebrandt, Petra; Jehmlich, Nico; Michalik, Stephan; Stanca, Sarmiza E.; Fritzsche, Wolfgang; Völker, Uwe; Schmidt, Frank
• Is Part Of: Cytometry. Volume 85:Issue 2(2014:Feb.)
• Page Start: 140
• Page End: 150
• Publication Date: 2013-12-17
• Subjects: Flow cytometry -- Periodicals; Imaging systems in biology -- Periodicals; Imaging systems in medicine -- Periodicals; Diagnostic imaging -- Periodicals; 571.605
• DOI: 10.1002/cyto.a.22425
• Languages: English
• ISSNs: 1552-4922

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>Throughout the world, infections caused by bacteria such as <italic>Staphylococcus aureus</italic> are a major cause of morbidity and mortality. In order to gain some understanding of the complicated physiological link between host and pathogen, modern techniques such as confocal microscopy and sophisticated OMICs technologies are suitable. However, labeling of pathogens such as <italic>S. aureus</italic> with green fluorescent protein, for example, or the generation of a reliable antibody, which are prerequisites for the application of reproducible isolation techniques, does not always succeed. Here, we present a universal approach for monitoring pathogen traffic after internalization into host cells by fluorescence microscopy and for isolation of bacteria from host–pathogen interaction assays using gold or ferric oxide‐core, poly(vinyl alcohol) coated, and fluorescence‐labeled nanoparticles (NP). The incubation of <italic>S. aureus</italic> HG001 with those NP had only minor effects on the bacterial growth <italic>in vitro</italic>. Quantitative proteome analysis after 24 h of NP incubation revealed that presence of NP provoked only marginal changes in the proteome pattern. The method presented enabled us to investigate the behavior of <italic>S. aureus</italic> HG001 during infection of S9 human epithelial cells by means of fluorescence microscopy and proteomics using magnetic separation or cell sorting. © 2013 International Society for Advancement of Cytometry</p> </abstract>