Dicer‐Processed Small RNAs: Rules and Exceptions1. Issue 1 (16th November 2012)

• Record Type: Journal Article
• Authors: Langenberger, David; Çakir, M. Volkan; Hoffmann, Steve; Stadler, Peter F.
• Is Part Of: Journal of experimental zoology. Volume 320:Issue 1(2013)
• Page Start: 35
• Page End: 46
• Publication Date: 2012-11-16
• Subjects: Developmental biology -- Periodicals; Evolution (Biology) -- Periodicals; Molecular evolution -- Periodicals; Zoology -- Periodicals; Evolution, Molecular -- Periodicals; Developmental Biology -- Periodicals; Zoology -- Periodicals; 591
• DOI: 10.1002/jez.b.22481
• Languages: English
• ISSNs: 1552-5007

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="jezb22481-sec-0001" sec-type="section"> <p>Canonical microRNAs are excised from their hairpin‐shaped precursors by <italic>Dicer</italic>. In order to find possible exceptions to this rule and to identify additional substrates for <italic>Dicer</italic> processing we re‐evaluate the small RNA sequencing data of the <italic>Dicer</italic> knockdown experiment in MCF‐7 cells orignally published by Friedländer et al. [Friedländer et al., 2012, Nucleic Acids Res 40:37–52]. While the well‐known non‐<italic>Dicer</italic> mir‐451 is not sufficiently expressed in these experiments, there are several additional <italic>Dicer</italic>‐independent microRNAs, among them the important tumor supressor mir‐663a. We recover previously described examples of non‐miRNA <italic>Dicer</italic> substrates such as tRNA‐Gln and several snoRNAs. Interestingly, sdRNAs derived from box C/D snoRNAs are <italic>Dicer</italic>‐independent, while those derived from box H/ACA snoRNAs are often <italic>Dicer</italic> dependent. Several pol‐III transcripts, in particular the vault RNAs and the great ape specific snaRs are processed by <italic>Dicer</italic>, while the small RNAs originating from Y RNAs seem to be <italic>Dicer</italic> independent. J. Exp. Zool. (Mol. Dev. Evol.) 320B:35–46, 2013. © 2012 Wiley Periodicals, Inc.</p> </sec> </abstract>