Mast cells can regulate skeletal muscle cell proliferation by multiple mechanisms. Issue 3 (27th June 2013)

• Record Type: Journal Article
• Authors: Duchesne, Elise; Bouchard, Patrice; Roussel, Marie‐Pier; Côté, Claude H.
• Is Part Of: Muscle & nerve. Volume 48:Issue 3(2013:Sep.)
• Page Start: 403
• Page End: 414
• Publication Date: 2013-06-27
• Subjects: Neuromuscular diseases -- Periodicals; Muscles -- Periodicals; Nerves -- Periodicals; 616.74
• DOI: 10.1002/mus.23758
• Languages: English
• ISSNs: 0148-639X

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p> <italic>Introduction</italic>: Mast cells (MCs) can stimulate cell proliferation, but their specific contribution to skeletal muscle regeneration is not well defined. <italic>Methods</italic>: L6 myoblast proliferation was assessed in coculture with MCs or when grown with MC‐conditioned media. To address the <italic>in vivo</italic> implication of MCs in regeneration, rats were treated with cromolyn, and myoblast proliferation, immune cell accumulation, and myogenic factors were assessed in bupivacaine‐injured muscles. <italic>Results</italic>: <italic>In vitro</italic>, both procedures increased the L6 cell proliferation rate, and this was tryptase‐dependent. <italic>In vivo</italic>, MC stabilization increased myoblast proliferation and accumulation of macrophages CD68 and CD163 after injury. This correlated with a sequential increase in MyoD and myogenin protein level expression. <italic>Conclusions</italic>: MCs can directly stimulate muscle cell proliferation via tryptase. MCs can influence myoblast proliferation <italic>in vivo, </italic> but this effect seems to be predominantly related to their modulation of macrophage recruitment. The MC is a potential actor in the early stages of muscle healing. <italic>Muscle Nerve</italic><bold>48</bold>: 403–414, 2013</p> </abstract>