Imaging the pH evolution of an acute kidney injury model by means of iopamidol, a MRI‐CEST pH‐responsive contrast agent. Issue 3 (11th October 2012)

• Record Type: Journal Article
• Authors: Longo, Dario Livio; Busato, Alice; Lanzardo, Stefania; Antico, Federica; Aime, Silvio
• Is Part Of: Magnetic resonance in medicine. Volume 70:Issue 3(2013:Sep.)
• Page Start: 859
• Page End: 864
• Publication Date: 2012-10-11
• Subjects: Nuclear magnetic resonance -- Periodicals; Electron paramagnetic resonance -- Periodicals; 616.07548
• DOI: 10.1002/mrm.24513
• Languages: English
• ISSNs: 0740-3194

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="mrm24513-sec-0001" sec-type="section"> <title>Purpose</title> <p>To investigate in vivo possible pH level alterations following an acute renal failure disease using a MRI‐CEST pH responsive contrast agent. The impact of functional evolution in different renal compartments over time was also investigated.</p> </sec> <sec id="mrm24513-sec-0002" sec-type="section"> <title>Methods</title> <p>a mouse model of acute kidney injury was obtained by glycerol‐induced rhabdomyolysis. pH maps were obtained using Iopamidol (0.75 g iodine/kg b.w. corresponding to 2.0 mmol/kg) in a control group (<italic>n</italic> = 3) and in the acute kidney injury group (<italic>n</italic> = 6) at 1, 3, 7, 14, and 21 days after the damage induction at 7T. Histology assessment of renal damage and blood urea nitrogen levels were compared with pH maps.</p> </sec> <sec id="mrm24513-sec-0003" sec-type="section"> <title>Results</title> <p>during the acute kidney injury, there was a robust increase of pH values, which peaked after 3 days, compared with the predamage situation. In addition, it was possible to detect changes in contrast detection between the different functional regions of the damaged kidneys. Moreover, a slow restoration of normal pH values was observed three weeks after the glycerol injection.</p> </sec> <sec id="mrm24513-sec-0004" sec-type="section"> <title>Conclusions</title> <p>pH appears to be a good parameter to assess the early detection of kidney injury as well as it acts as a reporter of the recovery toward the physiologic functionality. Magn Reson Med 70:859–864, 2013. © 2012 Wiley Periodicals, Inc.</p> </sec> </abstract>