Light‐Switchable Polymer from Cationic to Zwitterionic Form: Synthesis, Characterization, and Interactions with DNA and Bacterial Cells. Issue 8 (12th February 2013)

• Record Type: Journal Article
• Authors: Sobolčiak, Patrik; Špírek, Mário; Katrlík, Jaroslav; Gemeiner, Peter; Lacík, Igor; Kasák, Peter
• Is Part Of: Macromolecular rapid communications. Volume 34:Issue 8(2013:Apr.)
• Page Start: 635
• Page End: 639
• Publication Date: 2013-02-12
• Subjects: Macromolecules -- Periodicals; Polymers -- Periodicals; Chemistry -- Periodicals; 547.705
• DOI: 10.1002/marc.201200823
• Languages: English
• ISSNs: 1022-1336

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>A novel cationic polymer poly(<italic>N, N</italic>‐dimethyl‐<italic>N</italic>‐[3‐(methacroylamino) propyl]‐<italic>N</italic>‐[2‐[(2‐nitrophenyl)methoxy]‐2‐oxo‐ethyl]ammonium chloride) is synthesized by free‐radical polymerization of <italic>N</italic>‐[3‐(dimethylamino)propyl] methacrylamide and subsequent quaternization with <italic>o</italic>‐nitrobenzyl 2‐chloroacetate. The photolabile <italic>o</italic>‐nitrobenzyl carboxymethyl pendant moiety is transformed to the zwitterionic carboxybetaine form upon the irradiation at 365 nm. This feature is used to condense and, upon the light irradiation, to release double‐strand DNA tested by gel electrophoresis and surface plasmon resonance experiments as well as to switch the antibacterial activity to non‐toxic character demonstrated for <italic>Escherichia coli</italic> bacterial cells in solution and at the surface using the self‐assembled monolayers. <boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg1xr0s778" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>