Solanidine isolation from Solanum tuberosum by centrifugal partition chromatography. Issue 14 (15th July 2013)

• Record Type: Journal Article
• Authors: Attoumbré, Jacques; Giordanengo, Philippe; Baltora‐Rosset, Sylvie
• Is Part Of: Journal of separation science. Volume 36:Issue 14(2013:Jul.)
• Page Start: 2379
• Page End: 2385
• Publication Date: 2013-07-15
• Subjects: Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201300188
• Languages: English
• ISSNs: 1615-9306

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The aim of this investigation was the preparative isolation of solanidine (aglycone of the two main potato glycoalkaloids: α‐chaconine and α‐solanine) from fresh <italic>Solanum tuberosum</italic> (cv. Pompadour) material by implementing a new preparation scheme using centrifugal partition chromatography (CPC). A setup for obtaining solanidine by hydrolysis of the glycoalkaloids found in the skin and sprouts of <italic>S. tuberosum</italic> was first developed. Then its isolation was carried out by the development of CPC conditions: the solvent system used for separation was ethyl acetate/butanol/water in the ratio 42.5:7.5:50 v/v/v, 0.6 g of crude extract were separated with a 8 mL/min flow rate of mobile phase while rotating at 2500 rpm. A run yielded 98 mg of solanidine (86.7 % recovery from the crude extract) in a one‐step separation. The purity of the isolated solanidine was over 98%. Thus, CPC has proven to be the method of choice to get solanidine of very high purity from <italic>S. tuberosum</italic> biomass in large quantities.</p> </abstract>