Validated HPTLC method for the simultaneous quantification of diterpenoids in Vitextrifolia L. Issue 14 (21st June 2013)

• Record Type: Journal Article
• Authors: Tiwari, Neerja; Yadav, Akhilesh Kumar; Gupta, Madan Mohan
• Is Part Of: Journal of separation science. Volume 36:Issue 14(2013:Jul.)
• Page Start: 2373
• Page End: 2378
• Publication Date: 2013-06-21
• Subjects: Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201300131
• Languages: English
• ISSNs: 1615-9306

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <italic>Vitex trifolia</italic> L. is an important Indian medicinal plant with diverse pharmacological properties. In a recent study, we reported the isolation and antitubercular activity evaluation of three new diterpenoids from its leaves; here we have developed a validated rapid, simple, precise, and accurate high‐performance TLC method for the simultaneous quantification of isolated diterpenoids in <italic>V. trifolia</italic>. Diterpenoids, 6α, 7α‐diacetoxy‐13‐hydroxy‐8(9), 14‐labdadien (<bold>A</bold>), 13‐hydroxy‐5(10), 14‐halimadien‐6‐one (<bold>B</bold>), and 9‐hydroxy‐13(14)‐labden‐16, 15‐olide (<bold>C</bold>) were separated on silica gel 60F<sub>254</sub> high‐performance TLC plates using chloroform/acetone (98:2, v/v) as mobile phase. The quantitation of diterpenoids was carried out using densitometric reflection/absorption mode at 610 nm after postchromatographic derivatization using a vanillin/sulfuric acid reagent. A precise and accurate quantification can be performed for compounds <bold>A</bold> and <bold>B</bold> in the linear working concentration range of 333–1000 ng/band and for <bold>C</bold> in the range of 670–2000 ng/band with good correlations (<italic>r</italic> = 0.9984, 0.9991, and 0.9994, respectively). The method was validated for peak purity, precision, accuracy, robustness, LOD, and LOQ, as per the ICH guidelines. The method reported here is simple, reproducible and may be applied for the quantitative analysis of the above diterpenoids in the leaves of <italic>V. trifolia</italic>.</p> </abstract>