Evaluation of stationary phases based on silica hydride for the analysis of drugs of abuse. Issue 17 (4th September 2013)

• Record Type: Journal Article
• Authors: Pesek, Joseph J.; Matyska, Maria T.; Kim, Amy M.; Svec, Frantisek
• Is Part Of: Journal of separation science. Volume 36:Issue 17(2013:Sep.)
• Page Start: 2760
• Page End: 2766
• Publication Date: 2013-09-04
• Subjects: Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201300377
• Languages: English
• ISSNs: 1615-9306

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Several stationary phases based on silica hydride were evaluated as possible separation media for drugs of abuse. In the RP mode both a C₁₈ and a phenyl column were tested using ACN/water and methanol/water mobile phases with formic acid or ammonium formate as modifiers. Detection was performed using MS, so separation of isobaric species was a factor in selecting the column, mobile phase composition, and gradient. A number of sample preparation procedures were also included as part of determining the most appropriate experimental protocol on these types of stationary phases. Morphine was used as a model for developing a protocol that would be suitable for the analysis of hydrophilic drugs.</p> </abstract>