A rapid and convenient method for preparative separation of three indissolvable polyphenols from Euphorbia pekinensis by the flexible application of solvent extraction combined with counter‐current chromatography. Issue 14 (18th June 2013)

• Record Type: Journal Article
• Authors: Gu, Jiamin; Feng, Reihong; Wu, Zhiyuan; Guan, Shuguang; Guo, Jiling; Cheng, Chunru; Xu, Pingping; Zhang, Jingxian; Wang, Yang; Qu, Hua; Man, Wei; Yu, Wenbo; Cui, Yajun; Guan, Shuhong; Guo, Dean
• Is Part Of: Journal of separation science. Volume 36:Issue 14(2013:Jul.)
• Page Start: 2366
• Page End: 2372
• Publication Date: 2013-06-18
• Subjects: Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201300008
• Languages: English
• ISSNs: 1615-9306

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>A rapid and convenient method was established to preparatively isolate the three ellagic acid types of compounds, which were the main polyphenols in <italic>Euphorbia pekinensis</italic>, by flexibly applying solvent extraction combined with counter‐current chromatography (CCC). The total extract (extracted using 95% ethanol) of <italic>E</italic>. <italic>pekinensis</italic> was pretreated by two simple steps before CCC isolation, following the procedure: the total extract was extracted by classical solvent extraction using petroleum ether and ethyl acetate, respectively, and then the ethyl acetate extract was suspended using 95% ethanol, after being allowed to stand overnight, the sediment was obtained. Partial sediment (100 mg) was then directly separated by CCC with a two‐phase solvent system composed of chloroform‐95% ethanol‐water‐85% formic acid (50:50:50:5, v/v/v/v). About 22 mg of 3, 3′‐dimethoxy ellagic acid (1), 12 mg of 3, 3′‐di‐<italic>O</italic>‐methyl‐4‐<italic>O</italic>‐(<italic>β</italic>‐<sc>d</sc>‐xylopyranosyl)ellagic acid (2), and 35 mg of ellagic acid (3) with purities of 96.0, 95.2, and 95.4% were obtained respectively in one step within 4 h. After being purified by washing with methanol, the purities of the three compounds obtained were all above 98%. The purities were determined by HPLC and their chemical structures were further identified by <sup>1</sup>H and <sup>13</sup>C NMR spectroscopy. The recoveries were calculated as 84.6, 85.7, and 89.5%, respectively. The result demonstrated that the present isolation method was rapid, economical and efficient for the preparative separation of polyphenols from <italic>E. pekinensis</italic>.</p> </abstract>