Exploring nutritional modes of cultivation for enhancing lipid accumulation in microalgae. (26th June 2012)

• Record Type: Journal Article
• Authors: Ratha, Sachitra Kumar; Babu, Santosh; Renuka, Nirmal; Prasanna, Radha; Prasad, Rachapudi Badari Narayana; Saxena, Anil Kumar
• Is Part Of: Journal of basic microbiology. Volume 53:issue 5(2013:May)
• Page Start: 440
• Page End: 450
• Publication Date: 2012-06-26
• Subjects: Microbiology -- Periodicals; 579
• DOI: 10.1002/jobm.201200001
• Languages: English
• ISSNs: 0233-111X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="jobm201200001-sec-0001" sec-type="section"> <p>The objective of this study was to identify the most promising nutritional mode of growth for enhanced biomass and lipid productivity in a set of twenty microalgal strains, grown under photoautotrophic and mixotrophic/heterotrophic conditions using 2% glucose as carbon source. These included four cyanobacterial strains (<italic>Cyanosarcina</italic>, <italic>Phormidium</italic>, <italic>Nostoc</italic> and <italic>Anabaena</italic>) and sixteen green algae belonging to six genera (five strains each of <italic>Chlorella</italic> and <italic>Chlorococcum</italic>, two of <italic>Scenedesmus</italic> and one each of <italic>Chlamydomonas</italic>, <italic>Kirchneria</italic>, <italic>Bracteacoccus</italic> and <italic>Ulothrix</italic>). Lipid productivity ranged from 2–13% under photoautotrophic conditions, 1.7–32% under mixotrophic conditions and 0.9–20% under heterotrophic conditions. MIC‐G5 <italic>Chlorella</italic> sp. followed by MIC‐G11 <italic>Chlorella</italic> sp. exhibited the highest cellular lipid content (355 and 271 μg/ml) and lipid productivity of 32% and 28% respectively in mixotrophic condition. In the glucose supplemented conditions (heterotrophic), a significant reduction in PUFA from 25.1 to 9.4, 29.2 to 12.4 and 44.7 to 10.2 was observed in MIC‐G4, MIC‐G5 and MIC‐G11, respectively. A remarkable enhancement of 33–70% in SFA was recorded under mixotrophic conditions. As the quality of biodiesel is based on high SFA and low PUFA, our results illustrate the significance of glucose supplemented condition as a promising strategy for generating high value biodiesel from algae.</p> </sec> </abstract>