Edaphobacillus lindanitolerans gen. nov., sp. nov., isolated from hexachlorocyclohexane (HCH) contaminated soil1. (15th January 2013)

• Record Type: Journal Article
• Authors: Lal, Devi; Khan, Fazlurrahman; Gupta, Sanjay Kumar; Schumann, Peter; Lal, Rup
• Is Part Of: Journal of basic microbiology. Volume 53:issue 9(2013:Sep.)
• Page Start: 758
• Page End: 765
• Publication Date: 2013-01-15
• Subjects: Microbiology -- Periodicals; 579
• DOI: 10.1002/jobm.201200150
• Languages: English
• ISSNs: 0233-111X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="jobm201200150-sec-0001" sec-type="section"> <p>A Gram‐staining‐positive, aerobic, cocco‐bacilli‐shaped, non‐motile, non‐spore forming, cream colored strain bacterium (strain MNA4<sup>T</sup>) was isolated from hexachlorocyclohexane (HCH) contaminated soil. Strain MNA4<sup>T</sup> showed the highest 16S rRNA gene sequence similarity of 97.47% with type species of the newly defined genus <italic>Bhargavaea cecembensis</italic>. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MNA4<sup>T</sup> belonged to a clade represented by <italic>Bhargavaea cecembensis</italic>, <italic>Bacillus beijingensis</italic> and <italic>Bacillus ginsengi</italic>. DNA‐DNA hybridization values of the strain MNA4<sup>T</sup> with close relatives were well below the 70% threshold value recommended for delineation of species. The major fatty acids were anteiso C<sub>15:0</sub>, anteiso C<sub>17:0</sub>, iso C<sub>16:0</sub> and iso C<sub>15:0</sub>. The strain was found to contain respiratory quinones MK‐8, MK‐9 and MK‐7 in an approximate molar ratio of 85:7:5. Polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol and two unknown phospholipids. The DNA G + C content was 55.6 mol%. Peptidoglycan type was A4α (L‐Lys – L‐Ala – D‐Asp). Phylogenetic analysis, fatty acids profile, phenotypic properties and chemotaxonomic data of strain MNA4<sup>T</sup> indicated that it represents a novel species of a novel genus for which the name <italic>Edaphobacillus lindanitolerans</italic> gen. nov., sp. nov., is proposed. The type strain is MNA4<sup>T</sup> (=CCM 7584<sup>T</sup> = DSM 22424<sup>T</sup>).</p> </sec> </abstract>