Inactivation or clearance of Coxiella burnetii in rat serum samples to enable safe serological testing. (7th September 2012)

• Record Type: Journal Article
• Authors: Opsteegh, Marieke; de Heer, Lianne; van den Berg, Harold; van der Giessen, Joke
• Is Part Of: Journal of basic microbiology. Volume 53:issue 9(2013:Sep.)
• Page Start: 796
• Page End: 798
• Publication Date: 2012-09-07
• Subjects: Microbiology -- Periodicals; 579
• DOI: 10.1002/jobm.201200211
• Languages: English
• ISSNs: 0233-111X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jobm201200211-sec-0001" sec-type="section"> <p>To allow processing of serum samples from animals experimentally infected with <italic>Coxiella burnetii</italic> outside the BSL‐3 facility, an inactivation or clearance protocol that does not hamper serological testing may be required. The effects of filtration (0.1 µm pore size), heating at 56 °C for 30 min, addition of NaN₃ (0.09% w/v), and combinations thereof on the presence of viable <italic>C. burnetii</italic> as well as OD‐values in ELISA were tested. Only filtration was shown to effectively clear all culturable <italic>C. burnetii</italic>.</p> </sec> </abstract>