19‐VNTR loci used in genotyping Chinese clinical Mycobacterium tuberculosis complex strains and in association with spoligotyping. (15th January 2013)

• Record Type: Journal Article
• Authors: Jiang, Yi; Liu, Hai‐can; Zheng, Huajun; Dou, Xiangfeng; Tang, Biao; Zhao, Xiu‐qin; Zhu, Yongqiang; Lu, Bing; Wang, Shengyue; Dong, Hai‐yan; Zhang, Yuan‐yuan; Zhao, Guoping; Wan, Kanglin
• Is Part Of: Journal of basic microbiology. Volume 53:issue 7(2013:Jul.)
• Page Start: 562
• Page End: 580
• Publication Date: 2013-01-15
• Subjects: Microbiology -- Periodicals; 579
• DOI: 10.1002/jobm.201200230
• Languages: English
• ISSNs: 0233-111X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jobm201200230-sec-0001" sec-type="section"> <p>Recently, tandem repeat typing has emerged as a rapid and easy method for the molecular epidemiology of the <italic>Mycobacterium tuberculosis (M. tuberculosis)</italic> complex. In this study, a collection of 19 VNTRs incorporating 15 previously described loci and 4 newly evaluated markers were used to genotype 206 Chinese <italic>M. tuberculosis</italic> isolates and 9 BCG strains. The discriminatory power was evaluated and compared with that obtained by Spoligotyping. It turned out that 15‐locus VNTR could be very useful in <italic>M. tuberculosis</italic> complex strains genotyping in China. The 4 newly evaluated loci were proved informative and could be useful for future epidemiology studies, especially in Beijing family strains. In addition, a unique pattern of the latter 4 loci were found in Chinese BCG strains.</p> </sec> </abstract>