Anoxybacillus kaynarcensis sp. nov., a moderately thermophilic, xylanase producing bacterium. (26th June 2012)

• Record Type: Journal Article
• Authors: Inan, Kadriye; Belduz, Ali Osman; Canakci, Sabriye
• Is Part Of: Journal of basic microbiology. Volume 53:issue 5(2013:May)
• Page Start: 410
• Page End: 419
• Publication Date: 2012-06-26
• Subjects: Microbiology -- Periodicals; 579
• DOI: 10.1002/jobm.201100638
• Languages: English
• ISSNs: 0233-111X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="jobm201100638-sec-0001" sec-type="section"> <p>A novel moderately thermophilic, Gram‐positive, endospore‐forming, rod‐shaped, motile bacteria and alkaline active xylanase producing strain D1021<sup>T</sup>, was isolated from Kaynarca hot spring in the province of Izmir, Turkey and was characterized in order to determine its phylogenetic position. Growth was observed at 35–70 °C (optimum 60 °C), at pH 6.0–10.0 (optimum pH 7.0). The strain D1021<sup>T</sup> grew on a wide range of carbon sources including ribose, xylose, glucose, maltose, sucrose, arabinose, and melibiose. The major isoprenoid quinone was MK‐7. 16S rRNA gene sequence analysis showed that strain D1021<sup>T</sup> is related to members of genus <italic>Anoxybacillus</italic>, with similarities to <italic>Anoxybacillus</italic> spp. varying from 94.7 to 98.7. The major fatty acids of strain D1021<sup>T</sup> were iso‐C<sub>15:0</sub> (57.46%) and iso‐C<sub>17:0</sub> (13.98%). The DNA G + C content was 42.9 mol %. DNA–DNA relatedness values between <italic>Anoxybacillus</italic> spp. and D1021<sup>T</sup> were lower than 70%. On the basis of phenotypic characteristics, <italic>rpoB</italic> analysis, phylogenetic and DNA–DNA hybridization data, it was proposed that the strain D1021<sup>T</sup> (= DSM 21706<sup>T</sup> = LMG 25303<sup>T</sup>) should be placed in the genus <italic>Anoxybacillus</italic> as the type strain of a novel species, <italic>Anoxybacillus kaynarcensis</italic> sp. nov. The GenBank accession number for the 16S rRNA sequence is EU926955.</p> </sec> </abstract>