Pyrroloquinoline quinone and a quinoprotein kinase support γ‐radiation resistance in Deinococcus radiodurans and regulate gene expression. (7th September 2012)

• Record Type: Journal Article
• Authors: Rajpurohit, Yogendra Singh; Desai, Shruti Sumeet; Misra, Hari Sharan
• Is Part Of: Journal of basic microbiology. Volume 53:issue 6(2013:Jun.)
• Page Start: 518
• Page End: 531
• Publication Date: 2012-09-07
• Subjects: Microbiology -- Periodicals; 579
• DOI: 10.1002/jobm.201100650
• Languages: English
• ISSNs: 0233-111X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>ABSTRACT</title> <sec id="jobm201100650-sec-0001" sec-type="section"> <p> <italic>Deinococcus radiodurans</italic> is known for its extraordinary resistance to various DNA damaging agents including γ‐radiation and desiccation. The <italic>pqqE:cat</italic> and Δ<italic>dr2518</italic> mutants making these cells devoid of pyrroloquinoline quinone (PQQ) and a PQQ inducible Ser/Thr protein kinase, respectively, became sensitive to γ‐radiation. Transcriptome analysis of these mutants showed differential expression of the genes including those play roles in oxidative stress tolerance and (DSB) repair in <italic>D. radiodurans</italic> and in genome maintenance and stress response in other bacteria. <italic>Escherichia coli</italic> cells expressing DR2518 and PQQ showed improved resistance to γ‐radiation, which increased further when both DR2518 and PQQ were present together. Although, profiles of genes getting affected in these mutants were different, there were still a few common genes showing similar expression trends in both the mutants and some others as reported earlier in <italic>oxyR</italic> and <italic>pprI</italic> mutant of this bacterium. These results suggested that PQQ and DR2518 have independent roles in γ‐radiation resistance of <italic>D. radiodurans</italic> but their co‐existence improves radioresistance further, possibly by regulating differential expression of the genes important for bacterial response to oxidative stress and DNA damage.</p> </sec> </abstract>