An efficient protocol for isolating melanised chaetothyrialean anamorphic fungi associated with plant‐ants. (14th May 2012)

• Record Type: Journal Article
• Authors: Ruiz‐González, Mario X.; Lauth, Jérémie; Leroy, Céline; Jauneau, Alain; Gryta, Hervé; Jargeat, Patricia; Dejean, Alain; Orivel, Jérôme
• Is Part Of: Journal of basic microbiology. Volume 53:issue 1(2013:Jan.)
• Page Start: 98
• Page End: 100
• Publication Date: 2012-05-14
• Subjects: Microbiology -- Periodicals; 579
• DOI: 10.1002/jobm.201100539
• Languages: English
• ISSNs: 0233-111X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="jobm201100539-sec-0001" sec-type="section"> <p>Because of their ecological characteristics, slow growth rates and the presence of contaminants, Chaetothyriales fungi associated with structures built by tropical plant‐ants can be difficult to isolate with standard procedures. Here, we describe an easy‐to‐use protocol for obtaining pure cultures by using cotton as a first substrate. We have further found by means of fluorescent stains that nuclei concentrate either in young hyphae or in the tips of the hyphae.</p> </sec> </abstract>