Non‐trisomic homeobox gene expression during craniofacial development in the Ts65Dn mouse model of Down syndrome. Issue 8 (10th July 2013)

• Record Type: Journal Article
• Authors: Billingsley, Cherie N.; Allen, Jared R.; Baumann, Douglas D.; Deitz, Samantha L.; Blazek, Joshua D.; Newbauer, Abby; Darrah, Andrew; Long, Brad C.; Young, Brandon; Clement, Mark; Doerge, R.W.; Roper, Randall J.
• Is Part Of: American journal of medical genetics. Volume 161:Issue 8(2013:Aug.)
• Page Start: 1866
• Page End: 1874
• Publication Date: 2013-07-10
• Subjects: Medical genetics -- Periodicals; 616.14205
• DOI: 10.1002/ajmg.a.36006
• Languages: English
• ISSNs: 1552-4825

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="ajmga36006-sec-0001" sec-type="section"> <p>Trisomy 21 in humans causes cognitive impairment, craniofacial dysmorphology, and heart defects collectively referred to as Down syndrome. Yet, the pathophysiology of these phenotypes is not well understood. Craniofacial alterations may lead to complications in breathing, eating, and communication. Ts65Dn mice exhibit craniofacial alterations that model Down syndrome including a small mandible. We show that Ts65Dn embryos at 13.5 days gestation (E13.5) have a smaller mandibular precursor but a normal sized tongue as compared to euploid embryos, suggesting a relative instead of actual macroglossia originates during development. Neurological tissues were also altered in E13.5 trisomic embryos. Our array analysis found 155 differentially expressed non‐trisomic genes in the trisomic E13.5 mandible, including 20 genes containing a homeobox DNA binding domain. Additionally, <italic>Sox9</italic>, important in skeletal formation and cell proliferation, was upregulated in Ts65Dn mandible precursors. Our results suggest trisomy causes altered expression of non‐trisomic genes in development leading to structural changes associated with DS. Identification of genetic pathways disrupted by trisomy is an important step in proposing rational therapies at relevant time points to ameliorate craniofacial abnormalities in DS and other congenital disorders. © 2013 Wiley Periodicals, Inc.</p> </sec> </abstract>