MRT letter: Localization of endogenous hydrogen peroxide by modified processes of sample preparation for transmissionelectron microscope in Escherichia coli. Issue 2 (15th November 2012)

• Record Type: Journal Article
• Authors: Li, Xin; Hu, Rongliu; Zhu, Wenxue; Fan, Jinling; Pang, Xinyue; Wang, Na; Wang, Liping; Yang, Lipeng; Zhao, Chunyan; He, Chenyang
• Is Part Of: Microscopy research and technique. Volume 76:Issue 2(2013:Feb.)
• Page Start: 121
• Page End: 125
• Publication Date: 2012-11-15
• Subjects: Electron microscopy -- Technique -- Periodicals; Microscopy -- Periodicals; Microscopy -- Technique -- Periodicals; 502.825
• DOI: 10.1002/jemt.22152
• Languages: English
• ISSNs: 1059-910X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>The bacterial endogenous hydrogen peroxide (H₂O₂) was detected cytochemically by its reaction with cerium chloride (CeCl₃) to produce electron‐dense deposits of cerium perhydroxides. The sequence of fixation and CeCl₃ staining of H₂O₂ in the processing of transmission electron microscope (TEM) sample preparation is crucial to the localization of endogenous H₂O₂ in <italic>Escherichia coli</italic>. In this study, results confirmed that the process that fixation simultaneously with CeCl₃ staining provided optimum effects for H₂O₂ localization in <italic>E. coli</italic>. The modified process of TEM provides very efficient protection for H₂O₂ localization and more accurate quantization for the H₂O₂ accumulation in bacterial cells. Microsc. Res. Tech., 2013. © 2012 Wiley Periodicals, Inc.</p> </abstract>