Comparative evaluation of a new chemiluminiscent assay and an ELISA for the detection of IgM against measles. Issue 6 (November 2013)

• Record Type: Journal Article
• Authors: Sampedro, Antonio; Rodríguez‐Granger, Javier; Gómez, Cristina; Lara, Ana; Gutierrez, José; Otero, Ana
• Is Part Of: Journal of clinical laboratory analysis. Volume 27:Issue 6(2013:Nov.)
• Page Start: 477
• Page End: 480
• Publication Date: 2013-11
• Subjects: Diagnosis, Laboratory -- Periodicals; Medical laboratory technology -- Periodicals; 616
• DOI: 10.1002/jcla.21630
• Languages: English
• ISSNs: 0887-8013

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jcla21630-sec-0010" sec-type="section"> <title>Background</title> <p>The primary test for the laboratory confirmation of measles is immunoglobulin M (IgM) serology. It is therefore important to evaluate new commercial measles IgM immunoassays to ensure high‐quality measles diagnostic testing. The purpose of this study was to evaluate the diagnostic performance of LIAISON IgM measles (DiaSorin, Saluggia, Italy), a new automated chemiluminescence immunoassay (CLIA), in comparison with enzyme immunoassay (EIA) Enzygnost (Siemens, Marburg, Germany).</p> </sec> <sec id="jcla21630-sec-0020" sec-type="section"> <title>Methods</title> <p>Sensitivity was examined using a panel of measles laboratory confirmed sera; specificity was addressed by testing sera from healthy populations and subjects with infections that may interfere with measles IgM serology.</p> </sec> <sec id="jcla21630-sec-0030" sec-type="section"> <title>Results</title> <p>The diagnostic performances of the two assays were very similar: both Enzygnost EIA and LIAISON CLIA performed with a sensitivity of 93.7% and 98.8%, whereas the specificity was 96.8% and 97.9%, respectively.</p> </sec> <sec id="jcla21630-sec-0040" sec-type="section"> <title>Conclusion</title> <p>We concluded that LIAISON IgM measles can be a good alternative to the other immunoassay for laboratory measles confirmation.</p> </sec> </abstract>