Reduced Plasma Citrulline Levels in Low Birth Weight Infants With Necrotizing Enterocolitis. Issue 4 (12th July 2013)

• Record Type: Journal Article
• Authors: Celik, Istemi Han; Demirel, Gamze; Canpolat, Fuat Emre; Dilmen, Ugur
• Is Part Of: Journal of clinical laboratory analysis. Volume 27:Issue 4(2013:Jul.)
• Page Start: 328
• Page End: 332
• Publication Date: 2013-07-12
• Subjects: Diagnosis, Laboratory -- Periodicals; Medical laboratory technology -- Periodicals; 616
• DOI: 10.1002/jcla.21607
• Languages: English
• ISSNs: 0887-8013

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jcla21607-sec-0010" sec-type="section"> <title>Background</title> <p>Citrulline, a nonprotein amino acid, is an intermediate of the urea cycle and synthesized in small intestine. Lower plasma citrulline levels were associated with reduced function of enterocytes. Necrotizing enterocolitis (NEC) causes high morbidity and mortality, and leads impaired intestinal functions.</p> </sec> <sec id="jcla21607-sec-0020" sec-type="section"> <title>Methods</title> <p>Plasma citrulline levels of neonates with a gestational age <32 weeks and ≤1, 500 gm who developed NEC stage II/III were measured by high‐performance liquid chromatography.</p> </sec> <sec id="jcla21607-sec-0030" sec-type="section"> <title>Results</title> <p>We enrolled 36 preterm infants including 20 with NEC and 16 controls. Median citrulline levels of NEC and control groups were 8.6 and 20.18 μmol/l (<italic>P</italic> < 0.05), and cut off level of citrulline was 13.15 μmol/l with a sensitivity of 80% and a specificity of 82%. Median arginine levels of NEC and control groups were 22.02 and 39.89 μmol/l (<italic>P</italic> < 0.05), and cut off level of arginine was 28.52 μmol/l with a sensitivity of 70% and a specificity of 75%. Blood sampling day, gender, parenteral, and enteral nutrition did not affect the amino acid levels.</p> </sec> <sec id="jcla21607-sec-0040" sec-type="section"> <title>Conclusion</title> <p>We found lower plasma citrulline and arginine levels in preterm infants with NEC. Further studies are needed to determine most appropriate levels to predict recovery and prognosis of NEC, and treatment options with these amino acids in preterm infants.</p> </sec> </abstract>