Fragile X AGG analysis provides new risk predictions for 45–69 repeat alleles1. Issue 4 (26th February 2013)

• Record Type: Journal Article
• Authors: Nolin, Sarah L.; Sah, Sachin; Glicksman, Anne; Sherman, Stephanie L.; Allen, Emily; Berry‐Kravis, Elizabeth; Tassone, Flora; Yrigollen, Carolyn; Cronister, Amy; Jodah, Marcia; Ersalesi, Nicole; Dobkin, Carl; Brown, W. Ted; Shroff, Raghav; Latham, Gary J.; Hadd, Andrew G.
• Is Part Of: American journal of medical genetics. Volume 161:Issue 4(2013:Apr.)
• Page Start: 771
• Page End: 778
• Publication Date: 2013-02-26
• Subjects: Medical genetics -- Periodicals; 616.14205
• DOI: 10.1002/ajmg.a.35833
• Languages: English
• ISSNs: 1552-4825

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>We investigated the effect of AGG interruptions on fragile X repeat instability upon transmission of fragile X intermediate and small premutation alleles with 45–69 CGG repeats. The <italic>FMR1</italic> repeat structure was determined for 375 mothers, 48 fathers, and 538 offspring (457 maternal and 81 paternal transmissions) using a novel PCR assay to determine repeat length and AGG interruptions. The number of AGG interruptions and the length of uninterrupted CGG repeats at the 3′ end were correlated with repeat instability on transmission. Maternal alleles with no AGGs conferred the greatest risk for unstable transmissions. All nine full mutation expansions were inherited from maternal alleles with no AGGs. Furthermore, the magnitude of repeat expansion was larger for alleles lacking AGG interruptions. Transmissions from paternal alleles with no AGGs also exhibited greater instability than those with one or more AGGs. Our results demonstrate that characterization of the AGG structure within the <italic>FMR1</italic> repeat allows more accurate risk estimates of repeat instability and expansion to full mutations for intermediate and small premutation alleles. © 2013 Wiley Periodicals, Inc.</p> </abstract>